What a good way to start 2019: pubmed has reliably informed me that our methods article on using Super-Resolution microscopy to study HCV has just been published. Our work can be found alongside contributions from the great and good of HCV research in the latest volume of Methods in Molecular Biology, edited by Mansun Law. A browse through the contents section makes it clear that this book will be an invaluable resource for HCV research in the coming years: everything is covered from bioinformatics to immunology.
For our part, we provided detailed step-by-step instructions for performing state-of-the-art super-resolution microscopy of infected cells. I wrote the article with Pedro and Caron from Ricardo Henriques’ Lab, who provided their (seriously impressive) microscopy expertise. The approach we outlined uses the SRRF (pronounced ‘surf’) algorithm developed by the Henriques lab; this is particularly attractive as it is able to extract super-resolution images even with comparatively basic microscopes. Also, the protocol we provide is not limited to HCV, so can be applied to lots of biological systems; why don’t you give it a try.